Recent sexual violence: initial tests, follow-up and drug-facilitated assault

• Less than 48 hours: inform about the existence of PEP and arrange without delay a consultation at the emergency department or the referral service; the tests must not delay PEP. Offer emergency contraception (copper IUD effective up to 5 days).

Laboratory tests

• β-hCG (woman of childbearing potential without contraception)if neededWoman of childbearing potential without contraception, at the first visit and whenever she wishes ^[2].
• HIV serology
• Hepatitis B serology: HBsAg, anti-HBs, anti-HBc (unless immunity documented or status already known)
• Hepatitis C serology
• Syphilis serology
• Chlamydia trachomatis and gonococcus NAAT (PCR), 1 site (self-collected vaginal sample or first-catch urine)
• Chlamydia trachomatis and gonococcus NAAT (PCR), 2 sites (plus anal or oral penetration)if neededOne site per exposed area: pharynx after oral-genital contact, anus after anal intercourse ^[2]; a single billing code according to the number of sites ^[3].
• Chlamydia trachomatis and gonococcus NAAT (PCR), 3 sites (genital, anal and pharyngeal)if neededGenital, anal and oral-genital exposure ^[2]; replaces the 1-site or 2-site NAATs (PCR) ^[3].
• ALT (if PEP)if neededOnly if PEP is prescribed ^[2].
• Serum creatinine with eGFR (if PEP)if neededOnly if PEP is prescribed ^[2].

At 6 weeks (without PEP), 10 weeks (after PEP) or 12 weeks (risk of hepatitis)

• HIV serology
• Syphilis serology
• Chlamydia trachomatis and gonococcus NAAT (PCR) on the exposed site(s)
• Hepatitis B serology: HBsAg, anti-HBs, anti-HBc (non-immune victim; at 12 weeks)if neededVictim not immune to HBV: check at 12 weeks ^[2].
• Hepatitis C serologyif needed“Hepatitis C risk”: traumatic anal practice, source who injects drugs, chemsex, source living with untreated HCV ^[2].
• ALT (traumatic anal penetration or at-risk assailant; at 12 weeks)if needed“Hepatitis C risk”: traumatic anal practice, source who injects drugs, chemsex, source living with untreated HCV ^[2].
• β-hCG (woman of childbearing potential without contraception)if neededWoman of childbearing potential without contraception, whenever she wishes ^[2].

• Pilot scheme (since January 2026, for 3 years) in Hauts-de-France, Île-de-France and Pays de la Loire: tests covered without a complaint being filed. Write “protocole SC” on the prescription; give the information leaflet, the liaison form (2 copies) and the CRAFS consent form (01 40 05 42 70).

Events dating from 5 days ago or less: as soon as possible

• Search for substances that may impair consciousness or judgement, on blood and urine (2 lithium heparin tubes, 2 EDTA tubes, 2 fluoride tubes; 2 urine bottles without preservative)
• An early lock of hair (to distinguish habitual use from a single exposure)if needednon-French guidelineWhen habitual exposure (treatment, consumption) must be distinguished from a single exposure, add an early hair sample within 5 days ^[7,8].

Events dating from more than 5 days ago, or repeated events: between 1 and 6 months after the events

• Search for substances on 4 locks of hair (posterior vertex, cut close to the scalp)